Nucleotide Analysis
Nucleotides are monomers to oligonucleotides, such as DNA and RNA, encoding genes and transcripts. Phosphorylated forms of nucleotides, such as ATP and GTP, are used as cofactors for enzymatic reactions or for signaling.
This analysis covers over 20 nucleotides and modified nucleotides.
Minimal sample amounts:
- Bodily Fluids (serum/plasma, urine, etc.): 25 μL (50 μL preferred)
- Cells: 1e6 cell pellets
- Tissues: 20 mg (50 mg preferred)
- Feces: 30 mg
- Analyzing nucleotides of DNA or RNA from oligonucleotides:
5-25 μg of DNA/RNA should be digested using P1 nuclease and/or DNase I.
The digest should then be further treated with Antarctic Phosphatase
and Phosphodiesterase I to obtain individual nucleotides. Contact us for more guidance. - Other sample sources contact us for more guidance
Below is a list of the nucleotides and modified nucleotides covered in this panel. Don't see your favorite nucleotide of interest? Let us know and we'll add it to the list when analyzing your samples.